Instrumentation
Waters Select Series Cyclic Ion Mobility-Mass Spectrometer
The lab has a state-of-the-art cyclic ion mobility-mass spectrometer (cIM-MS) capable of IM^n separations, and IM-selection. The cIM-MS is equipped with a nanoelectrospray ionization source, a 32,000 m/z quadrupole, and a ~1 meter cyclic ion mobility "racetrack" optimized for analysis of high-mass biomolecular analytes. Three activation regions (labeled 1, 2, 3) enable collision induced unfolding/dissociation, with the added capability of performing surface induced dissociation in activation region 1. The time-of-flight detector has a resolution greater than 100,000 FWHM, for reliable, and accurate mass measurement.
Thermo Scientific Q Exactive Ultra High Mass Range Mass Spectrometer
The lab has a state-of-the-art ultra high mass range (UHMR) orbitrap mass spectrometer. The UHMR will be with equipped with a nanoelectrospray ionization source, a 25,000 m/z quadrupole, 200,000 m/z mass range, and a high resolution orbitrap mass detector capable of achieving a resolution of 200,000 FWHM at 400 m/z. Additionally, our UHMR will be equipped with Direct Mass Technology Mode enabling charge detection mass spectrometry, or "individual ion mass spectrometry" capabilities, substantially extending the mass range while improving sensitivity and resolution.
Waters Synapt G2 - Quadrupole Ion Mobility Time-of-Flight Mass Spectrometer
The lab will have a previous generation ion mobility-mass spectrometer (IM-MS) equipped with a nanoelectrospray ionization source, a 32,000 m/z quadrupole, and a linear IM cell optimized for analysis of high-mass biomolecular analytes. This instrument is equipped with two activation regions (Trap, and Transfer) which can be operated with different collision gases to improve collisional activation of highly adducted, or structurally stabilized analytes. This instrument will also be equipped with future modifications to develop unique gas phase activation workflows.