Characterization of Structurally Challenging Biomolecules

by Native Mass Spectrometry

Investigation of Transient IDP Oligomers

Structural information about toxic soluble oligomers of intrinsically disordered proteins (IDPs) could yield insight into the mechanisms and rates of IDP aggregation, a hallmark of various degenerative diseases. Native mass spectrometry (nMS) is capable of analyzing transient oligomeric states of IDPs and capturing the structural ensembles they form enroute to aggregation. nMS, and gas phase activation will be applied to investigate mixed IDP aggregation, and time-resolved nMS methods will be developed to measure rates of IDP aggregation, and screen for potential inhibitors of IDP aggregation.

Addressing a Shortage of Nucleic Acid Structural Information

Nucleic acids prove to be challenging targets for existing structural analysis techniques, necessitating the development of novel approaches capable of elucidating structural information about these structurally enigmatic biomolecules. Novel nMS-based methods, including charge detection-MS, will be developed to make structural measurements of nucleic acids, and their complexes.

Bioanalytical Toolbox for Structural Mass Spectrometry

We use a variety of MS-based techniques to study biological targets of interest. In addition to biochemical discovery, we are continuously innovating around our core methodologies to enable analysis of emerging systems of interest. Learn more about the MS methods being applied and innovated in the Gadkari Research Group.

Native Mass Spectrometry | Ion Mobility | Ion Mobility-Mass Spectrometry | Electrospray Ionization | Charge Detection Mass Spectrometry

Gas Phase Activation Methods for Biomolecular Structure Characterization

Gas phase activation is a central component of studying biomolecular structure by mass spectrometry. We employ collision induced dissociation (CID), and surface induced dissociation (SID) to elucidate critical information about biomolecular complex structure, assembly, sub-structure, and sequence.

Gas Phase Structural Stability | Surface Induced Dissociation | Gas Phase Unfolding